1x nebuffer 2 (New England Biolabs)
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1x nebuffer 2
1x Nebuffer 2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2845 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+nebuffer+2/NEBuffer+2/bio_rxiv__64898__2026__02__25__708054-208-20-21
Average 99 stars, based on 2845 article reviews
1x Nebuffer 2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2845 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+nebuffer+2/NEBuffer+2/bio_rxiv__64898__2026__02__25__708054-208-20-21
Average 99 stars, based on 2845 article reviews
1x nebuffer 2 - by Bioz Stars,
2026-09
99/100 stars
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other:Article Title: Growth factor-independent mTORC1 signaling promotes primary cilia length via suppression of autophagy Article Snippet: The retroviral plasmid expressing GFP-LC3-RFP (pMRX-IP-GFP-LC3-RFP) was a gift from Noboru Mizushima (Addgene plasmid #84573; http://n2t.net/addgene:84573 ). Article Title: A Plastic EMP1 + to LGR5 + Cell State Conversion as a Bypass to KRAS G12D Pharmacologic Inhibition in Metastatic Colorectal Cancer Article Snippet: The dissociation was quenched with 3 mL of warm Advanced DMEM/F12 (Gibco, 12634028), supplemented with 10mM HEPES (Gibco, 11560496) and Glutamax (Gibco, 11574466). Produced:Article Title: Quantification of the effects of single nucleotide variants in NKX2.1 transcription factor binding sites Article Snippet: The PC was the unvaried thyroglobulin promoter sequence and the NC was a length-matched exonic sequence of the FOXA1 gene that was negative for NKX2.1 binding (for full sequences used please see Supplementary Table S1: oligos 6–7 ). .. Double-stranded DNA was produced using a Klenow enzyme with a reverse primer-only, specific to the mutant libraries ( Supplementary Table S1: oligo 8 ), the negative control ( oligo 10 ), or the positive control ( oligo 12 ). ssDNA template was incubated with 0.2 mM each of dATP, dCTP, dTTP and dGTP, 12 μM reverse primer either unlabelled or labelled with Alexa Fluor 647 dye in Mutagenesis:Article Title: Quantification of the effects of single nucleotide variants in NKX2.1 transcription factor binding sites Article Snippet: The PC was the unvaried thyroglobulin promoter sequence and the NC was a length-matched exonic sequence of the FOXA1 gene that was negative for NKX2.1 binding (for full sequences used please see Supplementary Table S1: oligos 6–7 ). .. Double-stranded DNA was produced using a Klenow enzyme with a reverse primer-only, specific to the mutant libraries ( Supplementary Table S1: oligo 8 ), the negative control ( oligo 10 ), or the positive control ( oligo 12 ). ssDNA template was incubated with 0.2 mM each of dATP, dCTP, dTTP and dGTP, 12 μM reverse primer either unlabelled or labelled with Alexa Fluor 647 dye in Negative Control:Article Title: Quantification of the effects of single nucleotide variants in NKX2.1 transcription factor binding sites Article Snippet: The PC was the unvaried thyroglobulin promoter sequence and the NC was a length-matched exonic sequence of the FOXA1 gene that was negative for NKX2.1 binding (for full sequences used please see Supplementary Table S1: oligos 6–7 ). .. Double-stranded DNA was produced using a Klenow enzyme with a reverse primer-only, specific to the mutant libraries ( Supplementary Table S1: oligo 8 ), the negative control ( oligo 10 ), or the positive control ( oligo 12 ). ssDNA template was incubated with 0.2 mM each of dATP, dCTP, dTTP and dGTP, 12 μM reverse primer either unlabelled or labelled with Alexa Fluor 647 dye in Positive Control:Article Title: Quantification of the effects of single nucleotide variants in NKX2.1 transcription factor binding sites Article Snippet: The PC was the unvaried thyroglobulin promoter sequence and the NC was a length-matched exonic sequence of the FOXA1 gene that was negative for NKX2.1 binding (for full sequences used please see Supplementary Table S1: oligos 6–7 ). .. Double-stranded DNA was produced using a Klenow enzyme with a reverse primer-only, specific to the mutant libraries ( Supplementary Table S1: oligo 8 ), the negative control ( oligo 10 ), or the positive control ( oligo 12 ). ssDNA template was incubated with 0.2 mM each of dATP, dCTP, dTTP and dGTP, 12 μM reverse primer either unlabelled or labelled with Alexa Fluor 647 dye in Incubation:Article Title: Quantification of the effects of single nucleotide variants in NKX2.1 transcription factor binding sites Article Snippet: The PC was the unvaried thyroglobulin promoter sequence and the NC was a length-matched exonic sequence of the FOXA1 gene that was negative for NKX2.1 binding (for full sequences used please see Supplementary Table S1: oligos 6–7 ). .. Double-stranded DNA was produced using a Klenow enzyme with a reverse primer-only, specific to the mutant libraries ( Supplementary Table S1: oligo 8 ), the negative control ( oligo 10 ), or the positive control ( oligo 12 ). ssDNA template was incubated with 0.2 mM each of dATP, dCTP, dTTP and dGTP, 12 μM reverse primer either unlabelled or labelled with Alexa Fluor 647 dye in Article Title: Growth factor-independent mTORC1 signaling promotes primary cilia length via suppression of autophagy Article Snippet: .. The lentiviral plasmid lentiCRISPRv2 (pLentiCRISPRv2), a gift from Feng Zhang (puromycin selection; Addgene plasmid # 52961; http://n2t.net/addgene:52961 ), or for targeting p53 (neomycin selection, lentiCRISPRv2-neo; Addgene; 98292), was modified by inserting the following sgRNA sequences at the BsmB1 site, with inserts prepared by annealing two primers, each containing a flanking sequence recognizing the BsmB1-digested lentiCRISPRv2 plasmid (sgRNA sequences in uppercase, Bsmb1-annealing sequences in lowercase): TSC1 5′-caccgGAGGAGCCGCCTGTTCCGGA-3’ 5′-aaacTCCGGAACAGGCGGCTCCTCc-3’ TSC2 5′-caccgGGAGAAGTCGCTCCACGCGG-3’ 5′-aaacCCGCGTGGAGCGACTTCTCCc-3’ TBC1D7 5′-caccgGCATGGGACTCGTGGTGTGG-3’ 5′-aaacCCACACCACGAGTCCCATGCc-3’ TP53 5′-caccgGAAGGGACAGAAGATGACAG-3’ 5′-aaacCTGTCATCTTCTGTCCCTTCc-3’ NPRL2 5′-caccgGGGCATCACACACGAAGCCC-3’ 5′-aaacGGGCTTCGTGTGTGATGCCCc-3’ ULK1 5′-caccgGCAGCGTCTGAGACTTGGCG-3’ 5′-aaacCGCCAAGTCTCAGACGCTGCc-3’ BECN1 5′-caccgGAAACCAGGAGAGACCCAGG-3’ 5′-aaacCCTGGGTCTCTCCTGGTTTCc-3’ ATG7 5′-caccgATAGCTGGGCAGCAACGGGC-3’ 5′-aaacGCCCGTTGCTGCCCAGCTATc-3’ TFEB 5′- caccgGCAGCAGTCGCAGCATCAGA-3’ 5′- aaacTCTGATGCTGCGACTGCTGCc-3’ To anneal primers (Integrated DNA Technologies), 100 μM of each were incubated in Purification:Article Title: Quantification of the effects of single nucleotide variants in NKX2.1 transcription factor binding sites Article Snippet: The PC was the unvaried thyroglobulin promoter sequence and the NC was a length-matched exonic sequence of the FOXA1 gene that was negative for NKX2.1 binding (for full sequences used please see Supplementary Table S1: oligos 6–7 ). .. Double-stranded DNA was produced using a Klenow enzyme with a reverse primer-only, specific to the mutant libraries ( Supplementary Table S1: oligo 8 ), the negative control ( oligo 10 ), or the positive control ( oligo 12 ). ssDNA template was incubated with 0.2 mM each of dATP, dCTP, dTTP and dGTP, 12 μM reverse primer either unlabelled or labelled with Alexa Fluor 647 dye in Agarose Gel Electrophoresis:Article Title: Quantification of the effects of single nucleotide variants in NKX2.1 transcription factor binding sites Article Snippet: The PC was the unvaried thyroglobulin promoter sequence and the NC was a length-matched exonic sequence of the FOXA1 gene that was negative for NKX2.1 binding (for full sequences used please see Supplementary Table S1: oligos 6–7 ). .. Double-stranded DNA was produced using a Klenow enzyme with a reverse primer-only, specific to the mutant libraries ( Supplementary Table S1: oligo 8 ), the negative control ( oligo 10 ), or the positive control ( oligo 12 ). ssDNA template was incubated with 0.2 mM each of dATP, dCTP, dTTP and dGTP, 12 μM reverse primer either unlabelled or labelled with Alexa Fluor 647 dye in Polymerase Chain Reaction:Article Title: Gene therapy via CRISPR/Cas9-mediated Cxcr4 disease allele inactivation reverses leukopenia in WHIM mice Article Snippet: .. The PCR products were diluted 1:4 in Plasmid Preparation:Article Title: Growth factor-independent mTORC1 signaling promotes primary cilia length via suppression of autophagy Article Snippet: .. The lentiviral plasmid lentiCRISPRv2 (pLentiCRISPRv2), a gift from Feng Zhang (puromycin selection; Addgene plasmid # 52961; http://n2t.net/addgene:52961 ), or for targeting p53 (neomycin selection, lentiCRISPRv2-neo; Addgene; 98292), was modified by inserting the following sgRNA sequences at the BsmB1 site, with inserts prepared by annealing two primers, each containing a flanking sequence recognizing the BsmB1-digested lentiCRISPRv2 plasmid (sgRNA sequences in uppercase, Bsmb1-annealing sequences in lowercase): TSC1 5′-caccgGAGGAGCCGCCTGTTCCGGA-3’ 5′-aaacTCCGGAACAGGCGGCTCCTCc-3’ TSC2 5′-caccgGGAGAAGTCGCTCCACGCGG-3’ 5′-aaacCCGCGTGGAGCGACTTCTCCc-3’ TBC1D7 5′-caccgGCATGGGACTCGTGGTGTGG-3’ 5′-aaacCCACACCACGAGTCCCATGCc-3’ TP53 5′-caccgGAAGGGACAGAAGATGACAG-3’ 5′-aaacCTGTCATCTTCTGTCCCTTCc-3’ NPRL2 5′-caccgGGGCATCACACACGAAGCCC-3’ 5′-aaacGGGCTTCGTGTGTGATGCCCc-3’ ULK1 5′-caccgGCAGCGTCTGAGACTTGGCG-3’ 5′-aaacCGCCAAGTCTCAGACGCTGCc-3’ BECN1 5′-caccgGAAACCAGGAGAGACCCAGG-3’ 5′-aaacCCTGGGTCTCTCCTGGTTTCc-3’ ATG7 5′-caccgATAGCTGGGCAGCAACGGGC-3’ 5′-aaacGCCCGTTGCTGCCCAGCTATc-3’ TFEB 5′- caccgGCAGCAGTCGCAGCATCAGA-3’ 5′- aaacTCTGATGCTGCGACTGCTGCc-3’ To anneal primers (Integrated DNA Technologies), 100 μM of each were incubated in Selection:Article Title: Growth factor-independent mTORC1 signaling promotes primary cilia length via suppression of autophagy Article Snippet: .. The lentiviral plasmid lentiCRISPRv2 (pLentiCRISPRv2), a gift from Feng Zhang (puromycin selection; Addgene plasmid # 52961; http://n2t.net/addgene:52961 ), or for targeting p53 (neomycin selection, lentiCRISPRv2-neo; Addgene; 98292), was modified by inserting the following sgRNA sequences at the BsmB1 site, with inserts prepared by annealing two primers, each containing a flanking sequence recognizing the BsmB1-digested lentiCRISPRv2 plasmid (sgRNA sequences in uppercase, Bsmb1-annealing sequences in lowercase): TSC1 5′-caccgGAGGAGCCGCCTGTTCCGGA-3’ 5′-aaacTCCGGAACAGGCGGCTCCTCc-3’ TSC2 5′-caccgGGAGAAGTCGCTCCACGCGG-3’ 5′-aaacCCGCGTGGAGCGACTTCTCCc-3’ TBC1D7 5′-caccgGCATGGGACTCGTGGTGTGG-3’ 5′-aaacCCACACCACGAGTCCCATGCc-3’ TP53 5′-caccgGAAGGGACAGAAGATGACAG-3’ 5′-aaacCTGTCATCTTCTGTCCCTTCc-3’ NPRL2 5′-caccgGGGCATCACACACGAAGCCC-3’ 5′-aaacGGGCTTCGTGTGTGATGCCCc-3’ ULK1 5′-caccgGCAGCGTCTGAGACTTGGCG-3’ 5′-aaacCGCCAAGTCTCAGACGCTGCc-3’ BECN1 5′-caccgGAAACCAGGAGAGACCCAGG-3’ 5′-aaacCCTGGGTCTCTCCTGGTTTCc-3’ ATG7 5′-caccgATAGCTGGGCAGCAACGGGC-3’ 5′-aaacGCCCGTTGCTGCCCAGCTATc-3’ TFEB 5′- caccgGCAGCAGTCGCAGCATCAGA-3’ 5′- aaacTCTGATGCTGCGACTGCTGCc-3’ To anneal primers (Integrated DNA Technologies), 100 μM of each were incubated in Modification:Article Title: Growth factor-independent mTORC1 signaling promotes primary cilia length via suppression of autophagy Article Snippet: .. The lentiviral plasmid lentiCRISPRv2 (pLentiCRISPRv2), a gift from Feng Zhang (puromycin selection; Addgene plasmid # 52961; http://n2t.net/addgene:52961 ), or for targeting p53 (neomycin selection, lentiCRISPRv2-neo; Addgene; 98292), was modified by inserting the following sgRNA sequences at the BsmB1 site, with inserts prepared by annealing two primers, each containing a flanking sequence recognizing the BsmB1-digested lentiCRISPRv2 plasmid (sgRNA sequences in uppercase, Bsmb1-annealing sequences in lowercase): TSC1 5′-caccgGAGGAGCCGCCTGTTCCGGA-3’ 5′-aaacTCCGGAACAGGCGGCTCCTCc-3’ TSC2 5′-caccgGGAGAAGTCGCTCCACGCGG-3’ 5′-aaacCCGCGTGGAGCGACTTCTCCc-3’ TBC1D7 5′-caccgGCATGGGACTCGTGGTGTGG-3’ 5′-aaacCCACACCACGAGTCCCATGCc-3’ TP53 5′-caccgGAAGGGACAGAAGATGACAG-3’ 5′-aaacCTGTCATCTTCTGTCCCTTCc-3’ NPRL2 5′-caccgGGGCATCACACACGAAGCCC-3’ 5′-aaacGGGCTTCGTGTGTGATGCCCc-3’ ULK1 5′-caccgGCAGCGTCTGAGACTTGGCG-3’ 5′-aaacCGCCAAGTCTCAGACGCTGCc-3’ BECN1 5′-caccgGAAACCAGGAGAGACCCAGG-3’ 5′-aaacCCTGGGTCTCTCCTGGTTTCc-3’ ATG7 5′-caccgATAGCTGGGCAGCAACGGGC-3’ 5′-aaacGCCCGTTGCTGCCCAGCTATc-3’ TFEB 5′- caccgGCAGCAGTCGCAGCATCAGA-3’ 5′- aaacTCTGATGCTGCGACTGCTGCc-3’ To anneal primers (Integrated DNA Technologies), 100 μM of each were incubated in Sequencing:Article Title: Growth factor-independent mTORC1 signaling promotes primary cilia length via suppression of autophagy Article Snippet: .. The lentiviral plasmid lentiCRISPRv2 (pLentiCRISPRv2), a gift from Feng Zhang (puromycin selection; Addgene plasmid # 52961; http://n2t.net/addgene:52961 ), or for targeting p53 (neomycin selection, lentiCRISPRv2-neo; Addgene; 98292), was modified by inserting the following sgRNA sequences at the BsmB1 site, with inserts prepared by annealing two primers, each containing a flanking sequence recognizing the BsmB1-digested lentiCRISPRv2 plasmid (sgRNA sequences in uppercase, Bsmb1-annealing sequences in lowercase): TSC1 5′-caccgGAGGAGCCGCCTGTTCCGGA-3’ 5′-aaacTCCGGAACAGGCGGCTCCTCc-3’ TSC2 5′-caccgGGAGAAGTCGCTCCACGCGG-3’ 5′-aaacCCGCGTGGAGCGACTTCTCCc-3’ TBC1D7 5′-caccgGCATGGGACTCGTGGTGTGG-3’ 5′-aaacCCACACCACGAGTCCCATGCc-3’ TP53 5′-caccgGAAGGGACAGAAGATGACAG-3’ 5′-aaacCTGTCATCTTCTGTCCCTTCc-3’ NPRL2 5′-caccgGGGCATCACACACGAAGCCC-3’ 5′-aaacGGGCTTCGTGTGTGATGCCCc-3’ ULK1 5′-caccgGCAGCGTCTGAGACTTGGCG-3’ 5′-aaacCGCCAAGTCTCAGACGCTGCc-3’ BECN1 5′-caccgGAAACCAGGAGAGACCCAGG-3’ 5′-aaacCCTGGGTCTCTCCTGGTTTCc-3’ ATG7 5′-caccgATAGCTGGGCAGCAACGGGC-3’ 5′-aaacGCCCGTTGCTGCCCAGCTATc-3’ TFEB 5′- caccgGCAGCAGTCGCAGCATCAGA-3’ 5′- aaacTCTGATGCTGCGACTGCTGCc-3’ To anneal primers (Integrated DNA Technologies), 100 μM of each were incubated in |